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Cells were pelleted at 1000rpm, resuspended in PBS, and lysed by three freeze-thaw cycles using a dry ice ethanol bath

A better understanding of the cell and context dependent role of SLC7A11 under different conditions of nutrient availability (for example, glucose and glutamine limiting conditions) is needed ( Figure 2 )

High glucose, glucose fluctuation and carbonyl stress enhance brain microvascular endothelial barrier dysfunction: implications for diabetic cerebral microvasculature

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Behavioural and physiological aspects of stress and aggression in nonhuman primates