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Cleavage of the covalent acyl bond between Thr-381 of the enzyme and the C5 of the -glutamyl group is the rate-limiting step in the catalytic reaction

In both lines, FADS knockdown inhibited colony formation, increased cell death, and elevated ROS and lipid peroxidation levels (Supplementary Fig

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Supplementation with acetyl-L-carnitine (100-300 mg/kg) for several weeks restores these parameters toward youthful levels

Moreover, zinc supplementation has demonstrated the ability to enhance lysosomal function by activating TFEB-mediated lysosomal biogenesis and promoting autophagic flux 103

The rate of change during this phase depends heavily on baseline body composition, caloric intake, activity level, and adherence to protocol