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The combined SRB was dissolved with 100 l 10 mM Tris base buffer (pH 10.5) and absorbance was measured at 495 nm with a microtiter plate reader (BioTek, USA)

Obesity (Silver Spring) 20:197483 Schmelzer C, Lindner I, Rimbach G, Niklowitz P, Menke T, Dring F (2008) Functions of coenzyme Q10 in inflammation and gene expression

While liver biopsy remains the gold standard for fibrosis evaluation, its invasiveness and associated risks make it less suitable for routine clinical application [52]