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[25] Calls to improve the evidence base have been made, for example through increased use of anthropometric outcome measures [8], consensus and consistency in defining and measuring environmental factors and weight-related outcomes [14], [26] improved reporting of study methods [27], improved sensitivity and specificity in approaches being used (e.g., measures appropriate to the population of interest) [28], and use of more complete datasets (e.g., via ground-truthing using in-person [29] or virtual audits [30]

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The antigen was unstable in microbial systems and required rapid turnaround for immunogenicity testing

In 1898, the Dutch microbiologist Martinus Beijerinck independently replicated Ivanovsky's experiments and became convinced that the filtered solution contained a new form of infectious agent, which he named virus

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