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All ischaemiareperfusion experiments were performed in a double-blinded manner

Researchers measured secretion of four critical pregnancy-related hormonesprogesterone, estradiol, human hyperglycosylated chorionic gonadotropin (hCG), and human placental lactogen (hPL)alongside P2 7 receptor activation, a marker of cellular stress

SDS-PAGE and western blotting Cell samples were lysed in 20 mM HEPES (pH 7.4), 300 mM NaCl, 10 mM KCl, 1 mM MgCl 2 , 20% glycerol, and 1% Triton X-100

doi: 10.1080/15216540051081010