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Different laboratories may employ varying preparation methods depending on research use, analytical techniques, and experimental design

PMID: 25756278 This page explains vitamin B12 injection schedules for educational purposes only

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Use in elderly care: They are a simple and safe method to ensure B12 supply for seniors whose absorption capacity is often reduced

Allow to separate, draw off the lower layer into a second small separator, add 5 mL of 5 N sulfuric acid, shake well, and allow to separate completely (the complete separation of the layer may be facilitated by centrifuging): the separated upper layer is colorless or has no more color than a mixture of 0.15 mL of 0.10 N potassium permanganate and 250 mL of water