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the entire feed line downstream of the pump, as far as the injector, is made of steel, and steam-sterilised before use

The selection criteria of the study were constrained to the language English because of the language barrier, high cost of translation, and time efficiency

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Immunofluorescence against the astrocyte marker glial fibrillary acidic protein (GFAP) and the microglial marker ionized-calciumbinding adaptor molecule 1 (Iba-1) was evaluated in coronal cryosections of hippocampus (30 m thick) as previously reported (Ezquer et al., 2 , and the thickness ( z axis) was measured for each case