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The pF1-GSH1-lin-3XFLAG and pF1-GSH2-lin-3XFLAG constructs were modified from pF1-GSH1-nsc and pF1-GSH2-nsc constructs respectively

Agonists of Nuclear Factor Erythroid 2-Related Factor 2 Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor that interacts with Keap1 and is ubiquitinated by Cullin 3 in the cytoplasm prior to its degradation in the proteasome

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These fluid shifts activate hearing cells in the cochlea (outer and inner hair cells which are the cells responsible for converting the mechanical energy of sound waves into electrical energy) and cause electrical signals to travel from the cochlea to the brain, thereby giving rise to the perception of sound

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