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Date: February 14, 2003 Event: Third Annual Biology Student Research Symposium Location: Dickinson College Kelly Hill Willard Establishment of Kuku Community C'onservation Area (KCCA,) in Kenya: Biodiversity Evaluation, Tourism Evaluation and Stakeholder Considerations

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Metere A, Frezzotti F, Graves CE, Vergine M, De Luca A, Pietraforte D, Giacomelli L

Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours

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