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Genotyping PCR analysis Genomic DNA was extracted using the Quick gDNA Miniprep Kit (ZYMO Research, USA) followed by PCR amplification of the targeted region in the ABCC1 gene using LA Taq DNA Polymerase (Takara Bio, Japan) according to standard laboratory protocols

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In the case of skin injury, AMPs expression is highly up-regulated at the wound edge due to increased synthesis by keratinocytes and deposition from degranulation of recruited neutrophils, which indicates that AMPs are potential wound-healing stimulator

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Experimental studies injury type A In an experimental in-vivo rattail model, intervertebral discs were loaded for 1.5 hours with approximately 300% body weight (12.6 N) at 1 Hz