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After 24 h of stimulation, the cells were harvested, washed with PBS and stained with 3 M MitoSOX Red probe for 15 min at 37C

Interactions between cationic peptides and negatively charged oligonucleotides can enhance toxicity, affect pharmacokinetic and pharmacological properties, and limit the efficacy of these therapies

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This is the dose studied in every FDA registration trial and the dose carried forward in long term extension work

Zhang Y, Roh YJ, Han SJ, Park I, Lee HM, Ok YS, Lee BC, Lee S (2020) Role of selenoproteins in redox regulation of signaling and the antioxidant system: a review