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The BL21 transformants were plated on LB agar plate with kanamycin (KAN) (30 g/mL) and incubated overnight at 37 C

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It is available only through research chemical suppliers and some compounding pharmacies, where it is often labeled not for human use to comply with regulations Australia: Classified as a Schedule 4 prescription-only medicine, meaning it can only be legally obtained with a doctors prescription

These findings support a personalised approach, where absorption efficiency may be more relevant than absolute dosage