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Before data collection, the crystals were soaked in 25% PEG4000, 25% PEG400, 50 mM MgCl 2 , 100 mM Tris (pH 8.5), and 10% glycerol

We tested the contribution of the growth factors IGF1 and FGF2 (taken from this protocol) for application in collagen hydrogel (of note, the relevant receptors are expressed at the mRNA level)

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[19] Long-term medical management includes the use of diazoxide, somatostatin analogs, nifedipine, glucagon, recombinant IGF-I, glucocorticoids, human growth hormone, dietary intervention, or combinations of these therapies

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