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Interacting host allele and Mtb strain show distinct global transcriptomic effects in macrophages To experimentally test the plausibility of the identified host and Mtb genetic association, we assessed the responses of human macrophages from Peruvian donors with different rs3130660 genotypes to infection with g2g-L2 and nearest neighbor L2 Mtb strains, here called non-g2g-L2
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HeLa cells transfected with the indicated vectors were analyzed by immunofluorescence with anti-HA, anti-Calnexin, and anti-Tom20 antibodies to visualize the localization of HMOX1 mutant or HMOX2 mutant, ER, and mitochondria, respectively
Notably, sidG expression is completely absent after treatment with H 2 O 2
