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To determine the ideal sample size n for this study, Yamanes simplified sampling formula was used 18 : where, Z=1.96, was employed, assuming an unknown population proportion p set at 50% with a margin of error d set at 3.5%, resulting in an approximate requirement of 294 participants

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Besides, tobacco is rich in sugars (constituting 25%50% of dry weight) and proteins (26%29% of dry weight at the seedling stage and 12%15% of dry weight at the mature stage), as well as phytosterols such as -sitosterol, stigmasterol, and rape sterols

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