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Methotrexate slows down the progression [of inflammation] so it reduces the destruction of the joints. 2

and Looper, K.J., 2009

EDTA plasma rather than serum for B12, folate and ferritin estimations

Mast cells release histamine, prostaglandins, and other inflammatory mediators

While Zn supplementation has shown potential to improve outcomes in some cases (Table 1), variability in results highlights the need for further mechanistic understanding of the reciprocal mechanistic regulation between Zn metabolism and IBD