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Maintaining the overall ionic strength of the CFE environment is important to mimic the cellular environment and maintain the proper function of biological processes

We have shown that the glutathione pool is very insensitive to the large variations inacute amino acid input caused by daily meals

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For example, extracellular proteases, such as HGFA, matriptase, and MMP-2, can cleave and transform HGFs into their active biological state, thereby facilitating the release of HGF [55]

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The plates were incubated for 30 min at 25C before the addition of 100 l of the substrate solution (0.04 M Na 2 HPO 4 , 0.2 mm, 5,5-dithio-bis-2-nitrobenzoic acid (DTNB), 0.24 mm acethyltiocholine iodide (ATCI) in HPLC-grade water