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To conceptualize the biological basis of this process, fourteen hallmarks of ageing have been proposed, including genomic instability, telomere attrition, epigenetic alterations, impaired macroautophagy, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, altered intercellular communication, chronic inflammation, stem cell exhaustion, and dysbiosis [105]

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Angle the needle so the stream of water runs gently down the inside wall of the glass vial