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In addition to directly affecting the function of tumour cells themselves, inhibitors of the Wnt signalling pathway also promote the activation and infiltration of immune cells in the tumour microenvironment and assist in improving the tumour-killing effect
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BrdU incorporation was analysed by Biozero fluorescence microscopy (Keyence)
2.6 Immunophenotyping by flow cytometry PBMCs were isolated using Ficoll-Paque density gradient centrifugation, frozen in freezing medium [10% DMSO, 90% fetal bovine serum (FBS)] and stored at -80C until use
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