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They were then washed with PBS on the rotating bed for 5 min and permeabilized using 0.1% Triton X-100 in PBS for 10 min, followed by another 5 min PBS wash on the rotating bed

Shao, H

This highly invasive delivery methodology itself induces brain damage, introduces surgical risks from anesthesia and infection, and requires four separate cannulas to dose all essential terminal fields and cell body areas

Rats treated solely with rotenone exhibited significantly reduced mitochondrial respiratory function, including basal respiration, ATP production, and maximal respiration

Briefly, mice were anesthetized with isoflurane (5% induction, 1.52% maintenance) (CAS 26675-46-7, Laboratorios Karizoo, Spain)