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After weighing, tissues were instantly placed in BIOPS buffer (calcium-EGTA (10 mM), free calcium (0.1 M), imidazole (20 mM), taurine (20 mM), morpholineethanesulfonic acid (50 mM), dithiothreitol (DTT, 0.5 mM), MgCl 2 (6.56 mM), ATP 5.77 (mM), phosphocreatine (15 mM)

Guyer RD: Peer Review and Quality Assurance for Spine Surgery

J.PruvostC.PolleA.FoyerC

Since energy generation is so fundamental to all other processes, in some cases the word metabolism may refer more broadly to the sum of all chemical reactions in the cell