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Following resting for 24 h in serum-free RPMI-1640, differentiated THP-1 macrophages were indirectly co-cultured with 5 10 5 of GMSCs at a cell ratio of 1:2 (GMSC/THP-1 cells) that were seeded onto the top cell insert with 1-m-sized pores (Fisher Scientific) [39]

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Therefore, the values obtained by HPLC would be considered the most accurate, particularly considering that UV/Vis spectroscopic analysis can be highly dependent on the sample concentration, leading to an overestimation when using highly concentrated samples during measurements

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