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As a result, researchers can evaluate with a more complete research model rather than focusing on a single pathway

NAC is a direct precursor to cysteine, the rate-limiting amino acid in glutathione synthesis, and it reliably increases intracellular GSH levels across multiple tissues

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HeLa cells were co-transfected with pX458-gRNAs and the donor plasmid via Lipo3000, followed with G418 selection (800 g/mL)

doi: 10.3389/fimmu.2025.1622744